Journal of Remote Sensing Technology
Journal of Remote Sensing Technology(JRST)
ISSN:2330-1767(Print)
ISSN:2330-1775(Online)
Frequency: Annually
Website: www.bowenpublishing.com/jrst/
Finding the Spectral Signature of 15Nitrogen Isotopes in Plants by Hyperspectral Techniques
Abstract:
Expensive laboratory based instrumentation is typically used to perform stable isotope analysis, making sample run times lengthy and costly. Research was conducted to identify novel approaches to stable isotope analysis using infrared spectroscopy techniques in both laboratory and field based instrumentation. Buckwheat plants were grown in aeroponic conditions at three nitrogen concentration levels (0.32%, 0.20%, and 0.10%) of 95% 15N-labelled fertilizer solutions and compared to a control with natural abundance (0.0036%) nitrogen based fertilizer. Infrared instrumentation was used to measure the buckwheat leaves and show diagnostic wavelength shifts are correlated to their fertilizer concentration, occur at multiple infrared frequencies, and are associated with nitrogen functional group compounds present in the buckwheat. These measurements confirm that with remote sensing techniques, nitrogen isotopes are detectible in the infrared region. The ability to remotely sense nitrogen isotopes with either field or airborne sensors will enable faster analysis and wider spatial coverage than current techniques.
Keywords:Stable Isotopes; FTIR; Infrared Spectroscopy; Nitrogen
Author: Sonja K. Capelle,Stephen A. Macko

References:

  1. J. Hoefs, Stable Isotope Geochemistry, 6th ed., Berlin Heidelberg: Springer-Verlag, 2009.
  2. J. Coates, “Interpretation of infrared spectra, a practical approach,” Encyclopedia of Analytical Chemistry, R.A. Meyers, Ed. Chichester: John Wiley and Sons Ltd, 2000.
  3. S. Quillard, G. Louam, J.P. Buisson, M. Boyer, M. Lapkowski, A. Pron, and S. Lefrant, “Vibrational spectroscopic studied of the isotope effects in polyaniline,” Synthetic Metals, vol. 84, pp. 805-806, 1997.
  4. S.I. Yun, H.M. Ro, W.J. Choi, and S.X. Chang, “Interative effects of N fertilizer source and timing of fertilization leave specific N isotopic signatures in Chinese cabbage and soil,” Soil Biology and Biochemistry, vol. 38, pp. 1682-1689, 2006.
  5. M.A. Townsend, D. P. Young, and S. A. Macko, “Kansas case study applications of nitrogen-15 natural abundance method for identification of nitrate sources,” Jour. Haz. Substance Research, vol. 4, pp. 1-22, 2004.
  6. (2014) The Chesapeake Bay Foundation 2014 State of the Bay Report. [Online]. Available: http://www.cbf.org/.
  7. R. Jones, H. Ougham, H. Thomas, and S. Waalang, The Molecular Life of Plants, New Jersey: Wiley-Blackwell, 2013.
  8. L. Vernon and G. Seely, The Chlorophylls, New York: Academic Press Inc., 1966.
  9. S.A. Macko and N.E. Ostrom, “Pollution studies using stable isotopes,” Stable Isotopes in Ecology, K. Lajtha, and R. Michener, Ed. Oxford, United Kingdom: Blackwell Scientific Pub., 1994, pp. 42-65.
  10. https://www.asdi.com/products-and-services/fieldspec-spectroradiometers.
  11. http://www.agilent.com/en-us/products/ftir/ftir-compact-portable-systems/4100-exoscan-series-ftir-(handheld).
  12. https://www.thermofisher.com/order/catalog/product/912A0760?ICID=search-product.
  13. T. Slonecker, B. Haack, and S. Price, “Spectroscopic Analysis of Arsenic Uptake in Pteris Ferns,” Remote Sensing, vol. 1(4), pp. 644-675, 2009.
  14. C.D. Elvidge and C. Zhikang, “Comparison of broad-band and narrow-bandred and near-infrared vegetation indicies,” Remote Sensing of the Environment, vol. 54(1), pp. 38-48, 1995.